Journal: Annals of Biomedical Engineering

Article Title: Biocompatibility Assessment of a New Biodegradable Vascular Graft via In Vitro Co-culture Approaches and In Vivo Model

doi: 10.1007/s10439-016-1601-y

Figure Lengend Snippet: Morphological and cell viability/proliferation studies. SEM micrographs of TPU/ePTFE grafts and morphology of the isolated primary fibroblasts and macrophages seeded on coverslips as control and on TPU/ePTFE grafts in mono-cultures and co-culture models, after 21 days. (a) TPU graft, (b) adventitial surface of the TPU graft and close up of the surface, (c) cross-section and close up of the wall structure, (d) primary fibroblasts (e) primary macrophages cultivated on plastic coverslips, (f) fibroblast mono-culture, (g) macrophage mono-culture, (h) fibroblast-macrophage juxtacrine co-culture and (i) macrophages in the fibroblast-macrophage paracrine co-culture after 21 days in TPU grafts. (j) ePTFE graft, (k) cross-section and close up of the wall structure, (l) macrophage mono-culture, (m) fibroblast mono-culture, (n) fibroblast-macrophage juxtacrine co-culture and (o) macrophages in the fibroblast-macrophage paracrine co-culture after 21 days, in ePTFE grafts. (p-r) Proliferation of fibroblast cells at different time points (7, 14 and 21 days), on TPU and ePTFE grafts in (p) fibroblast mono-culture, (q) fibroblast-macrophage juxtacrine co-culture and (r) fibroblasts seeded on transwell membrane in fibroblast-macrophage paracrine co-culture models. Data represent mean ± S.D. ( n = 3 per time-point per group, technical replicates: 3). * p < 0.05, ** p < 0.001.

Article Snippet: For paracrine co-culture, 0.4 μ m pore transwell ® polyester membrane cell culture inserts (Corning, USA) for 24 well plates were utilized.

Techniques: Isolation, Control, Co-Culture Assay, Membrane

Journal: Annals of Biomedical Engineering

Article Title: Biocompatibility Assessment of a New Biodegradable Vascular Graft via In Vitro Co-culture Approaches and In Vivo Model

doi: 10.1007/s10439-016-1601-y

Figure Lengend Snippet: Cell distribution studies and quantification of the positive cells via confocal microscopy. CD68 (green) and CD163 (red) immunofluorescence staining of macrophage and fibroblast mono-cultures and fibroblast-macrophage juxtacrine (FM Juxtacrine CC) and paracrine co-cultures (FM Paracrine CC) in (a) TPU and (b) ePTFE grafts after 21 days. The nuclei of cells were counterstained with DAPI (blue). (c) Quantification of the CD68 and CD163 positive cells on TPU and ePTFE grafts after 21 days in macrophage (M) and fibroblast (F) mono-cultures and fibroblast-macrophage juxtacrine (FM JUX CC) and paracrine co-cultures (FM PAR CC). Data are presented as mean ± standard deviation ( n = 3 per group).

Article Snippet: For paracrine co-culture, 0.4 μ m pore transwell ® polyester membrane cell culture inserts (Corning, USA) for 24 well plates were utilized.

Techniques: Confocal Microscopy, Immunofluorescence, Staining, Standard Deviation

Journal: Annals of Biomedical Engineering

Article Title: Biocompatibility Assessment of a New Biodegradable Vascular Graft via In Vitro Co-culture Approaches and In Vivo Model

doi: 10.1007/s10439-016-1601-y

Figure Lengend Snippet: Early inflammatory gene expressions of TNF-α, IL-1α and IL-10 cytokines in the presence of TPU and ePTFE grafts in macrophage and fibroblast mono-cultures and macrophage-fibroblast juxtacrine (FM Juxtacrine CC) and paracrine co-culture (FM Paracrine CC) models, at different time points. The expression levels of all marker genes were normalized to the expression levels of GAPDH. Data are presented as mean ± standard deviation ( n = 3 per time-point per group, technical replicates: 3). (For better representation of the data, logarithmic scales have been used).

Article Snippet: For paracrine co-culture, 0.4 μ m pore transwell ® polyester membrane cell culture inserts (Corning, USA) for 24 well plates were utilized.

Techniques: Co-Culture Assay, Expressing, Marker, Standard Deviation

Journal: Annals of Biomedical Engineering

Article Title: Biocompatibility Assessment of a New Biodegradable Vascular Graft via In Vitro Co-culture Approaches and In Vivo Model

doi: 10.1007/s10439-016-1601-y

Figure Lengend Snippet: (a) CD68, CCR7 and CD163 macrophages gene expression studies in macrophage and fibroblast mono-cultures and fibroblast-macrophage juxtacrine (FM Juxtacrine CC) and paracrine co-culture (FM Paracrine CC) models, after 7, 14 and 21 days in TPU and ePTFE grafts. The expression levels of all marker genes were normalized to the expression levels of GAPDH. Data are expressed as mean ± SD ( n = 3 per time-point per group, technical replicates: 3). (b) The ratios of CCR7/CD163 are illustrated for TPU and ePTFE grafts for macrophages mono-cultures (M) and fibroblast-macrophage juxtacrine (FM JUX) and paracrine co-culture (FM PARA) models at different time points.

Article Snippet: For paracrine co-culture, 0.4 μ m pore transwell ® polyester membrane cell culture inserts (Corning, USA) for 24 well plates were utilized.

Techniques: Gene Expression, Co-Culture Assay, Expressing, Marker